Protective Effect of Methylsulfonylmethane in Caerulein-Induced Acute Pancreatitis and Associated Lung Injury in Mice
Methods: Male Swiss mice were treated with hourly intraperitoneal injections of caerulein (50 μg/kg) for 6 h. MSM (500 mg/kg) was administered intraperitoneally 1 h after the first caerulein injection (therapeutic). The serum amylase activity and myeloperoxidase (MPO) activity in lung and pancreas were measured. The levels of H2 S and interleukin (IL)-1β, cystathionine-γ-lyase (CSE) and CD34+ expressions in pancreas and lungs were determined by RT-PCR and ELISA.
Key findings: Methylsulfonylmethane significantly ameliorated pancreas and lung histopathological changes, decreased serum amylase, MPO activity and inhibited caerulein-induced IL-1β expression. Furthermore, MSM reduced caerulein-induced H2 S levels by alleviating the expression of CSE in pancreas and lungs and increased CD34 expression and inhibited nuclear factor (NF)-κB translocation in caerulein-induced AP and associated lung injury.
Conclusions: These findings indicate that MSM can effectively reduce inflammatory responses and induce the homing of CD34+ cells to the injured tissues.
Keywords: CD34; acute pancreatitis; hydrogen sulfide; lung injury; methylsulfonylmethane.
J Pharm Pharmacol . 2018 Sep;70(9):1188-1199. doi: 10.1111/jphp.12946. Epub 2018 Jul 3.