Study Title:

Adenovirus-36 Increases Abdominal Fat But Not Liver Fat

Study Abstract

AIMS: Infection with specific pathogens may lead to increased adiposity. The human adenovirus 36 (Ad36) is a relatively new factor in promoting adipogenesis. It seems to improve the metabolic profile, expanding adipose tissue and enhancing insulin sensitivity in animal models. The aim of this study was to investigate whether any association or predictor effect of Ad36 seropositivity is present in non-alcoholic fatty liver disease (NAFLD), a condition associated with obesity and insulin resistance (IR).

METHODS: Sixty-five NAFLD patients and 114 controls were investigated. Ultrasound bright liver score (BLS), body composition, IR evaluated by homeostasis model assessment of insulin resistance index (HOMA or HOMA-IR) and serum neutralization assay for antibodies to Ad36 were assessed.

RESULTS: Ad36-seropositive patients have a lower risk of bright liver [OR 0.505 (95% confidence interval (CI) 0.265-0.962)]; greater IR leads to a higher risk of bright liver [OR 9.673 (95% CI 4.443-21.058)]. Among NAFLD, Ad36-seropositive vs. Ad36-seronegative patients did not show a significant IR difference. Ad36-seropositive NAFLD patients, with the same levels of HOMA and BLS, had greater body mass index and body fat mass, in comparison with seronegative NAFLD patients. By a multiple linear regression model, BLS was explained by HOMA (beta 0.513; P<0.0001), high density lipoprotein cholesterol (beta-0.219, P<0.006) and Ad36 seropositivity (beta-0.202, P<0.005), Ad36 seropositivity did not explain HOMA in the other multiple logistic regression model.

CONCLUSIONS: Ad36 seropositivity is not associated with a significant difference of IR in NAFLD patients, but is associated with a greater adiposity. Ad36 seropositivity is associated with a lower occurrence of NAFLD and bright liver, which, conceivably, is not directly mediated by IR.

Comments by Atkinson:

In this issue, Trovato et al. (1) report the first evaluation of the association of non-alcoholic fatty liver disease (NAFLD) and human adenovirus-36 (Adv36). This evaluation is relevant because Adv36 infection has been associated with obesity in humans and animals (2–9). Obesity is known to be associated with an increased prevalence of NAFLD (10). Chickens, mice, rats and monkeys that were experimentally infected with Adv36 were shown to increase body fat, gain weight and have paradoxically reduced serum cholesterol and triglycerides (2–4). Adult humans in the US and Italy and children in Korea who have naturally contracted Adv36 infection are heavier than uninfected individuals (5–9). The pro-inflammatory cytokine, interleukin-6 (IL-6), has been reported to be increased in the liver of people with NAFLD and is thought to play a role in fat accumulation and insulin resistance (11). Bouwman et al. (12) reported increased IL-6 in human adipocytes in vitro, but there are no data in humans on presence of Adv36 in the liver or its effects in hepatocytes. Kapila et al. (13) found Adv36 DNA in the livers of experimentally infected hamsters along with a significantly altered lipoprotein metabolism. Based on these prior publications it might have been expected that individuals infected with Adv36 would have an increased prevalence of NAFLD. Surprisingly, Trovato et al. (1) found an inverse relationship between the two.

The authors studied 115 patients with a ‘bright liver score’ (BLS) on ultrasound indicative of NAFLD and 114 control subjects without liver disease. Fifty NAFLD patients were excluded because of factors including history of diabetes, hepatitis B or C, and liver enzyme assays above normal, leaving a total of 65 NAFLD patients in the analysis. Thus, this population is highly selected and represents those subjects with pure NAFLD in its mildest form. Adv36 infection was documented by detecting antibodies to Adv36 in the serum by serum neutralization assay. The prevalence of Adv36 antibodies in this population of NAFLD patients was 32% compared with an infection rate of 47% in those without NAFLD (1). Of the 179 total patients, 41% had been infected with Adv36 based on the presence of antibodies in serum. This paper is only the second study reporting the prevalence of Adv36 in adult humans and the authors found a much higher prevalence in Italy than has been reported in the US. Atkinson et al. (5) found that 30% of USA obese adults and 11% of non-obese adults had Adv36 antibodies.

Insulin resistance is known to be strongly associated with NAFLD, and obesity, particularly visceral obesity, is associated with insulin resistance (10). Trovato et al. (1) confirmed that in their total population, NAFLD patients had much higher insulin levels and insulin resistance than non-NAFLD patients. Because the liver appears protected by Adv36 infection in this study, the question becomes what are the mechanisms of the Adv36 protection from NAFLD? The analyses in this paper reveal that there is a complex relationship between Adv36 infection and insulin/insulin resistance and their associations with liver disease. Individuals, all of whom had NAFLD and also were positive for Adv36, had a higher body mass index (BMI) and fat mass than those who were Adv36 negative. However, serum glucose, total and HDL cholesterol, insulin, and insulin resistance by homoeostasis model-insulin resistance index (HOMA) were all similar between Adv36-positive and -negative patients with NAFLD. Thus, Adv36-infected NAFLD patients were heavier and fatter than non-Adv36 patients, but there were no differences in biochemical measures. This is in marked contrast to the situation in individuals all of whom were positive for Adv36, but did or did not have NAFLD. If NAFLD was present in Adv36 positive individuals, the serum insulin, HOMA insulin resistance and HDL cholesterol were highly significantly different than in those without NAFLD. This is similar to the situation in uninfected individuals: those with NAFLD also had much higher levels of insulin and insulin resistance and lower levels of HDL cholesterol than those without NAFLD.

Although the lower prevalence of NALFD in patients who were Adv36 positive might appear to be a paradoxical response based on prior reports of Adv36 induced obesity and increased IL-6 (12), there are data in the literature that may shed light on the mechanisms of this response. Pasarica et al. (14) showed that mice experimentally infected with Adv36 had significantly lower serum levels of several cytokines. Rogers et al. (15) found that experimental infection of human adipose tissue explants and human adipose-derived stem cells with Adv36 caused a ‘metabolically favourable’ response. Adv36 increased glucose transport and upregulated expressions of several proadipogenic genes, adiponectin and fatty acid synthase. It also reduced the expression of inflammatory cytokine macrophage chemoattractant protein-1 in a phosphotidylinositol 3-kinase (PI3K)-dependent manner. Fasting glucose and A1C levels were lower in Adv36-infected individuals than in non-infected matched controls (16). Wang et al. (16) found that Adv36-enhanced glucose uptake in vitro in diabetic and non-diabetic human skeletal muscle cells independent of insulin signalling via a Ras-activated PI 3-kinase pathway and Pasarica et al. (4) reported that Adv36 improved in vivo fasting serum insulin level and HOMA insulin sensitivity in rats. Because improved insulin sensitivity is associated with decreased hepatic lipid deposition, and the decreased serum cytokines presumably would have the same effect, it may not be so surprising that Adv36 infection would be associated with reduced NAFLD.

The paper of Trovato et al. (1) is an important first step into understanding hepatic metabolism with Adv36 infection, but many questions remain. The effects of Adv36 directly on hepatic glucose metabolism and hepatic glucose output, fatty acid synthesis and triglycerides formation within the liver, and the interplay of competing cytokines within the liver are just some of the questions that will need to be answered. There are two paradoxical observations in this study that will need to be elucidated. Of all patients with NAFLD, those with Adv36 infection are fatter and heavier than non-infected but do not have a higher insulin levels or insulin resistance. However, of all patients with Adv36 infection, those with NAFLD have a higher serum insulin and insulin resistance than those without. The mechanisms of insulin resistance in Adv36-positive patients with NAFLD need to be evaluated to determine whether they are dependent or independent of the NAFLD. The patients in this study were highly selected for very early or very mild NAFLD. Future studies should look at the prevalence of Adv36 infection in all subjects with NAFLD to determine both prevalence of infection and response of the liver in different situations. This study has opened a new avenue of research that may provide explanations for the spectrum of disease with NAFLD.

1Trovato GM, Martines GF, Garozzo A, et al. Ad36 adipogenic adenovirus in human non-alcoholic fatty liver disease. Liver Int 2010; 30: 184–90. Direct Link:AbstractFull Article (HTML)PDF(176K)References2Dhurandhar NV, Israel BA, Kolesar JM, et al. Increased adiposity in animals due to a human virus. Int J Obes Relat Metab Disord 2000; 24: 989–96. CrossRef,PubMed,ChemPort3Dhurandhar NV, Whigham LD, Abbott DH, et al. Human adenovirus Ad-36 promotes weight gain in male rhesus and marmoset monkeys. J Nutr 2002; 132: 3155–60. PubMed,ChemPort4Pasarica M, Shin AC, Yu M, et al. Human adenovirus 36 induces adiposity, increases insulin sensitivity, and alters hypothalamic monoamines in rats. Obesity 2006; 14: 1905–13. CrossRef,PubMed,ChemPort5Atkinson RL, Dhurandhar NV, Allison DB, et al. Human adenovirus-36 is associated with increased body weight and paradoxical reduction of serum lipids. Int J Obes 2005; 29: 281–6. CrossRef,PubMed6Pasarica M, Shin AC, Yu M, et al. Human adenovirus 36 induces adiposity, increases insulin sensitivity, and alters hypothalamic monoamines in rats. Obesity 2006; 14: 1905–13. CrossRef,PubMed,ChemPort7Atkinson RL, Lee I, Shin HJ, He J. Human adenovirus-36 antibody status is associated with obesity in children. Int J Pediatr Obes 2009; 10: 1–4. CrossRef8Trovato GM, Castro A, Tonzuso A, et al. Human obesity relationship with Ad36 adenovirus and insulin resistance. Int J Obes 2009 September 29 [Epub ahead of print]. CrossRef9Na HN, Hong YM, Kim J, et al. Association between human adenovirus-36 and lipid disorders in Korean schoolchildren. Int J Obes 2009 October 13 [Epub ahead of print]. 10Cheung O, Sanyal AJ. Recent advances in nonalcoholic fatty liver disease. Curr Opin Gastroenterol 2009; 25: 230–7. CrossRef,PubMed11Wieckowska A, Papouchado BG, Li Z, et al. Increased hepatic and circulating interleukin-6 levels in human nonalcoholic steatohepatitis. Am J Gastroenterol 2008; 103: 1372–9. Direct Link:12Bouwman JJ, Visseren FL, Bouter KP, Diepersloot RJ. Infection-induced inflammatory response of adipocytes in vitro. Int J Obes 2008; 32: 892–901. CrossRef,PubMed,ChemPort13Kapila M, Khosla P, Dhurandhar NV. Novel short-term effects of adenovirus Ad-36 on hamster lipoproteins. Int J Obes Relat Metab Disord 2004; 28: 1521–7. CrossRef,PubMed14Pasarica M, Loiler S, Dhurandhar NV. Acute effect of infection by adipogenic human adenovirus Ad36. Arch Virol 2008; 153: 2097–102. CrossRef,PubMed,ChemPort15Rogers PM, Mashtalir N, Rathod MA, et al. Metabolically favorable remodeling of human adipose tissue by human adenovirus type 36. Diabetes 2008; 57: 2321–31. CrossRef,PubMed16Wang ZQ, Cefalu WT, Zhang XH, et al. Human adenovirus type 36 enhances glucose uptake in diabetic and nondiabetic human skeletal muscle cells independent of insulin signaling. Diabetes 2008; 57: 1805–13. CrossRef,PubMed

Study Information

Trovato GM, Martines GF, Garozzo A, Tonzuso A, Timpanaro R, Pirri C, Trovato FM, Catalano D.
Ad36 adipogenic adenovirus in human non-alcoholic fatty liver disease.
Liver Int.
2010 February
Department of Internal Medicine, Clinica di Medicina Interna e Terapia Medica, Facoltà di Medicina e Chirurgia, Università di Catania, Catania, Italy.

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