Study Title:

Tocotrienols reduce nitric oxide release by LPS-stimulated microglia

Study Abstract

Anti-inflammatory actions of the vitamin E fragment tocotrienol have not been described for microglia. Here, we screened palm α-, γ- and δ-tocotrienol isoforms and Tocomin® 50% (contains spectrum of tocotrienols and tocopherols) for their ability to limit nitric oxide (NO) production by BV2 microglia. Microglia were treated with varying doses of tocotrienols for 24 h and stimulated with 1 μg/ml lipopolysaccharide (LPS). All tocotrienol isoforms reduced NO release by LPS-stimulated microglia, with 50 μM being the most potent tocotrienol dose. Of the isoforms tested, δ-tocotrienol lowered NO levels the most, reducing NO by approximately 50% at 48 h post-LPS treatment (p < .05). None of the tocotrienol doses tested affected microglia viability.

Study Information

Shi Wei Tan, Rajesh Ramasamy, Maha Abdullah, Sharmili Vidyadaran
Inhibitory effects of palm α-, γ- and δ-tocotrienol on lipopolysaccharide-induced nitric oxide production in BV2 microglia
Cellular Immunology
2014 January

Full Study

http://www.sciencedirect.com/science/article/pii/S0008874911001894