Bromelain Demonstrates Anti-Fungal Properties
Methods and Results: The purification of bromelain from pineapple stems was carried out by chromatography techniques and its antimicrobial activity was tested against the fungal pathogens F. verticillioides, F. oxysporum, and F. proliferatum by broth microdilution assay. A concentration of 0.3 μmol l(-1) of bromelain was sufficient for 90% growth inhibition of F. verticillioides. The capability of bromelain to inhibit fungal growth is related to its proteolytic activity.
Conclusions: The study demonstrated that stem bromelain exhibits a potent antifungal activity against phytopathogens and suggests its potential use as an effective agent for crop protection. Significance and Impact of the Study: The results support the use of a natural protease that accumulates at high levels in pineapple stems as alternative to the use of chemical fungicides for crop protection.
Belén LG, Martha H, Blanca SS.
Bromelain, a Cysteine Protease from Pineapple (Ananas Comosus) Stem, is Aa Inhibitor of Fungal Plant Pathogens.
Lett Appl Microbiol.
Department of Molecular Genetics. Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB. CRAG building, Campus UAB, Bellaterra (Cerdanyola del Vallés) 08193 Barcelona, Spain.